FAQs

Our FAQs aim to help our customers with frequently asked questions about our products and offer all round support. Please browse through these FAQs to find answers for the most commonly raised questions.

Installation questions

Installing a Stage

General instructions:

  1. When installing a stage you should ensure the stage is the correct model for the microscope. You can do this using the configurator and the model number of the stage (found on the label on the underside of the stage).
  2. Remove any previous stage and ensure there is maximum clearance for the stage, i.e., lower (raise) the condenser and if required remove the objectives. Turn the stage so that the “Prior” logo is at the front right (next to your right hand as you hold the stage). Angle the stage slightly away from you and place into position.
    1. For dovetail fixings, you will be require to tighten a screw to fix the stage (some are spring loaded and require pulling back to fit the stage).
    2. For screw fittings, hold the stage in place with one hand while you fix several screws to hold it in position. Ensure you have fitted all the screws before tightening each of them in turn.
    3. Push the stage (if possible) through it’s travel range to ensure no collisions with any microscope fittings. If required use changing limits to change the limits of travel on the stage.

My stage collides with my microscope:

  1. Use the following instructions to change the limits of your stage so that it no longer collides with the microscope. Please report your stage model and microscope make and model and a description of the problem to tech support.

Installing a Filter Wheel

General instructions:

  1. Ensure you have the correct adaptor for the microscope and position on the microscope, you can do this using the configurator. The adaptor part number will be on your dispatch note and on the packaging. Generally there are three positions which the filter wheel could be fitted:
    1. Bright field, the filter wheel will fit between the bright field light source and the sample.
    2. Emission, the filter wheel will fit between the sample and the camera port or/and eye pieces.
    3. Excitation, the filter wheel will fit between the excitation (Hg, Lumen, or other light source) and the microscope.
  2. How do I attach my adaptor to my filter wheel?
    1. The filter wheel is normally installed with the motor pointing away from the microscope. Look at the parts of the adaptor, one will mirror the connection on the microscope (part a), the other will fix to it (part b). You should attach part a to the side of the filter wheel with the motor on it, and part b to the flat side of the filter wheel. In some cases you may need to remove the filter wheel cover to attach the adaptor.
  3. How do I fit my filters?
    1. With your filter wheel there are supplied filter rings and a filter change tool.
    2. Please read filter change which explains how to install filters.

Installing a Shutter

General instructions:

  1. Ensure you have the correct adaptor for the microscope and position on the microscope, you can do this using the configurator. The adaptor part number will be on your dispatch note and on the packaging. Generally there are three positions which the shutter could be fitted:
    1. Bright field, the shutter will fit between the bright field light source and the sample
    2. Emission, the shutter will fit between the sample and the camera port or/and eye pieces.Excitation, the shutter will fit between the excitation (Hg, Lumen, or other light source) and the microscope.
  1. How do I attach my adaptor to my shutter?
    1. The shutter is normally installed with the silver side of the shutter blades pointing towards the light source. Look at the parts of the adaptor, one will mirror the connection on the microscope (part a), the other will fix to it (part b). You should attach part a to the side of the shutter with the black blades and part b to the side of the shutter with the silver blades. In some cases you may need to remove the shutter cover to attach the adaptor.

Installing a Focus Motor

General instructions:

  1. Standard focus motors can be installed onto most microscopes with a coarse and fine focus knob. The focus can be installed on the left or right side of the microscope, (the direction of turn of the motor can be reversed in the controller to compensate, see controller RS232 commands). Place the focus sleeve over the coarse focus, place the focus housing over the sleeve and fix with the grub screws. Push motor into the focus housing and gently push the pad onto the fine focus, fix the motor in position with the screw.

Fitting Instructions

  1. Fitting a standard H122. Focus motor with focus sleeve.
  2. Fitting a H122KON Olympus and Nikon direct couple kit.
  3. Fitting a H122LMZ for Leica MZ series.
  4. Fitting a H122N for Nikon E800.
  5. Fitting a H12215 for Nikon SMZ 1500.
  6. Fitting a H122X200 for Zeiss Axiovert 200.
  7. Fitting a H122AXIE.
  8. Fitting a H122AXIO.
  9. Fitting a H122LB.

If your motor is not in this list please contact tech support.

Installing a Lumen Fluorescence Illumination System

General instructions:

  1. To ensure your Lumen microscope adaptor is set up correctly – please view the Lumen Adaptors information sheet.
  2. To ensure your bulb is installed correctly, there are two different bulb fitting procedures – if you have purchased your unit prior to August 2007 please use this guide. If you have purchased your Lumen after August 2007 please use this guide.
  3. Ensure your Lumen 200 is installed with adequate airflow around it.
  4. Connecting my LumenPro 200.
    1. You can connect the Lumen 200PRO connections to the Filter 1, Filter 2 and 4th Axis (ProScan II/PCI II only).
    2. The variable aperture shutter can either be controlled via the Lumen 200PRO commandsor as a 10 position filter wheel.
    3. Changing LumenPro 200 Filters.

Purchasing questions

Can I use the Lumen with Cy5.5?

Yes, the Lumen 220 model is now available for Cy5.5.

Is the Lumen compatible with Cy5 and DAPI?

Yes, The standard Lumen 200 wavelength range is sufficient to cover both CY5 and DAPI.

Are custom light sources available?

Yes, We offer a service for larger quantities of light sources where we will design light sources for the purposes of our customers. Please see the OEM section of the website for more information on this, or contact tech support.

Do I need a NanoScanZ Piezo Z stage system?

The NanoScanZ is a high accuracy focus system designed for high speed fusion (z-stacking) techniques. The NanoScanZ system has up to 1nm resolution and can complete a move in 10 microseconds. Standard focus motors (with and without encoders) are capable of providing Z-stacking up to 0.5um thicknesses with good accuracy, but are somewhat slower than the NanoScanZ.

Why do we suggest an encoded focus for all Zeiss instruments?

An encoded focus is required if the microscope which is being used has a focus mechanism which is not directly connected to the fine focus system, e.g., most Zeiss models. In this case a Z-encoder is recommended to provide a reliable Z-position.