Optimize Z Stack Image Acquisition Speed Using ProScan III TTL Breakout Box (H31TTLBB)

05.01.14

This Application Note describes using a Master 8 pulse generator and Prior ProScan® III XYZ motor controller with Prior’s TTL Breakout Box, Shutter, and Z Motor to create a high-speed Z stack imaging system. Z-Stack imaging allows the user to combine several images taken at different focus depths to create one image with a greater depth of field than any one image. This technique is particularly useful when individual images have a very shallow depth of field. By using a ProScan® system, the process could be made much faster; and the user gained total control over the timing and movement of the system.